Crystallization and preliminary crystallographic studies of an antimicrobial protein from Pharbitis nil.

نویسندگان

  • S C Ha
  • K Min
  • J C Koo
  • Y Kim
  • D J Yun
  • M J Cho
  • K K Kim
چکیده

An antimicrobial protein from seeds of Pharbitis nil (Pn-AMP) which shows an antifungal activity towards several agriculturally important plant pathogens has been crystallized in the presence of equimolar N-acetylglucosamine with sodium citrate as precipitant. The crystal belongs to the hexagonal space group P6(1)22 (or P6(5)22), with unit-cell parameters a = b = 29.33 (5), c = 133.44 (12) A. Native data were collected using a crystal at 100 K to a resolution of 1.78 A.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Stress-Induced Flowering in Pharbitis—A Review

Many plant species are induced to flower by stress. Stress-induced flowering has been studied mostly in the short-day plant pharbitis (also called Japanese morning glory; Ipomoea nil, formerly Pharbitis nil). In this article, physiological characteristics, the regulation by salicylic acid (SA) and the expression of flowering-related genes in stress-induced flowering in pharbitis are reviewed. P...

متن کامل

Pharbinilic acid, an allogibberic acid from morning glory (Pharbitis nil).

Pharbinilic acid (1), the first naturally occurring allogibberic acid, was isolated from ethanol extracts of morning glory (Pharbitis nil) seeds. Its absolute configuration was determined by NOESY NMR and ECD experiments. Compound 1 showed weak cytotoxicity against A549, SK-OV-3, SK-MEL-2, and HCT-15 cells and weakly inhibited nitric oxide production in lipopolysaccharide-activated BV-2 microgl...

متن کامل

Preliminary X-ray crystallographic analysis of the glycosyltransferase from a marine Streptomyces species.

ElaGT is a glycosyltransferase from a marine Streptomyces species that is involved in the biosynthesis of elaiophylin. Here, the molecular cloning, protein expression and purification, preliminary crystallization and crystallographic characterization of ElaGT are reported. The rod-shaped crystals belonged to space group P2(1)22, with unit-cell parameters a=66.7, b=131.7, c=224.6 Å, α=90, β=90, ...

متن کامل

Purification and preliminary crystallographic studies of CutC, a novel copper homeostasis protein from Shigella flexneri.

CutC is a novel copper homeostasis protein containing 248 amino acids. Here we report the cloning, expression, purification, crystallization and preliminary X-ray crystallographic studies of CutC from Shigella flexneri 2a. Purification of CutC and its selenomethionine (SeMet) derivate were done using immobilized metal ion affinity chromatography, size-exclusion and ion-exchange chromatography. ...

متن کامل

Molecular cloning and expression analysis of a CONSTANS homologue, PnCOL1, from Pharbitis nil.

The Arabidopsis CONSTANS (CO) gene is a key regulator of the long day (LD)-dependent flowering pathway and two CO homologous genes COL1 and COL2 are involved in the regulation of the circadian rhythm. In order to understand the role of CO and COL in short-day plants, a CO homologue, PnCOL1, was isolated and characterized from Japanese morning glory (Pharbitis nil). The deduced PnCOL1 protein of...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Acta crystallographica. Section D, Biological crystallography

دوره 57 Pt 2  شماره 

صفحات  -

تاریخ انتشار 2001